Current Issue : January - March Volume : 2016 Issue Number : 1 Articles : 5 Articles
Wild-type p53 is well known to induce cell cycle arrest and apoptosis to block aberrant cell growth. However, p53�s unique role in\napoptosis and cell proliferation in Li-Fraumeni Syndrome (LFS) has not been well elucidated.The aim of this study is to characterize\nthe activity of wild-type p53 protein in LFS family dominated by a germline negative mutant p53. As expected, etoposide-treated\nwild-type p53-containing cell lines, LFS 2852 and control Jurkat, showed a greater rate of caspase- and annexin V-induced apoptotic\ncell death compared to the p53-mutant LFS 2673 cell line although mitochondrial and nuclear assays could not detect apoptosis in\nthese organelles.The most intriguing part of the observation was the abnormal proliferation rate of the wild-type p53-containing cell\nline,which grew twice as fast as 2673 and Jurkat cells. This is important because apoptosis inducers acting through the mitochondrial\ndeath pathway are emerging as promising drugs against tumors where the role of p53 is not only to target gene regulation but\nalso to block cell proliferation. This study casts a long shadow on the possible dysregulation of p53 mediators that enable cell\nproliferation. The deregulation of proliferation pathways represents an important anticancer therapeutic strategy for patients with\nthe LFS phenotype....
Ethnicity has been associated with the incidence of diseases and consequently it is a cornerstone\nin medical genetic studies. It is mainly important in admixture populations, where the population\nstratification can produce spurious results that lead to erroneous conclusions. Consequently, population\nstratification has become one of the most important confounding factors in populationbased\ngenetic association studies, especially in Latino populations. Cartagena de Indias is a cosmopolitan\ncity with dissimilar ancestry proportions due to recent miscegenation. This population\nmainly exhibits African and Amerindian matrilineal ancestries. Nevertheless, important asymmetries\nin the paternal genetic history related to the complex patterns of migration in the colonial\nperiod increase the male genetic diversity in this population. As a result of this recent admixture,\npopulation stratification has arisen, where each subpopulation is not equally represented. Consequently,\nthe allele differences between cases and controls could be related with different frequencies\namong different population strata rather than the association of the genes with the disease.\nIn order to define the patrilineal substructure of the Cartagena�s population, a total of 130\nunrelated men were ancestrally studied using 15 Y-STR loci routinely employed in anthropological,\nforensic and population genetics. Our results show that Cartagena is an admixture population\nconsisting of European (80%), Amerindian (10%) and African ancestries (10%), which are represented by haplogroups R1b and I2a (xI2a1), Q-M242/Q-M3, and E1b1a/E1b1b, respectively.\nComplex genetic patterns found in Cartagena�s population emphasize the importance to know the\ngenetic variation in order to diminish the in consistence for future genetic association studies. In\naddition, our findings illustrate the complex genetic background of Cartagena population and\nreinforce the need to encompass more geographic regions to generate more robust data for anthropological\nand forensic applications....
The CYP19A1 gene encodes the enzyme aromatase, which is responsible for the biosynthesis of estrogens. The rs 10046\npolymorphism of CYP19A1 gene has been investigated in two studies on the occurrence of hypertension, but there are no studies on\nits correlation with coronary artery disease (CAD).We investigated 189 subjects who were hospitalized at ââ?¬Å?KATââ?¬ÂGeneral Hospital of\nAthens and underwent coronary angiography. Of these, 123 were found with CAD with an average age of 60 years and constituted\nthe patients group and 66 subjects with an average age of 58 years without damage in the coronary vessels and constituted the\ncontrol group (healthy). The frequencies of genotypes CC, CT, and TT of rs10046 polymorphism are significantly different between\nthe group of CAD patients and the control group (0.34, 0.48, and 0.18 versus 0.20, 0.48, and 0.32, resp., ...
Aging is a complex process characterized by a steady decline in an organism�s ability to perform life-sustaining tasks. In the present\nstudy, two cages of approximately 12,000 mated Drosophila melanogaster females were used as a source of RNA from individuals\nsampled frequently as a function of age.Ali near model for microarray data method was used for the microarray analysis to adjust for\nthe box effect; it identified 1,581 candidate aging genes. Cluster analyses using a self-organizing map algorithm on the 1,581 significant\ngenes identified gene expression patterns across different ages. Genes involved in immune system function and regulation, chorion\nassembly and function, and metabolism were all significantly differentially expressed as a function of age. The temporal pattern\nof data indicated that gene expression related to aging is affected relatively early in life span. In addition, the temporal variance\nin gene expression in immune function genes was compared to a random set of genes. There was an increase in the variance of\ngene expression within each cohort, which was not observed in the set of random genes. This observation is compatible with the\nhypothesis that D. melanogaster immune function genes lose control of gene expression as flies age....
The number of sequenced crop genomes and associated genomic resources is growing rapidly with the advent of inexpensive next\ngeneration sequencing methods. Databases have become an integral part of all aspects of science research, including basic and\napplied plant and animal sciences. The importance of databases keeps increasing as the volume of datasets from direct and indirect\ngenomics, as well as other omics approaches, keeps expanding in recent years. The databases and associated web portals provide at\na minimum a uniform set of tools and automated analysis across a wide range of crop plant genomes. This paper reviews some basic\nterms and considerations in dealing with crop plant databases utilization in advancing genomic era. The utilization of databases for\nvariation analysis with other comparative genomics tools, and data interpretation platforms are well described.The major focus of\nthis review is to provide knowledge on platforms and databases for genome-based investigations of agriculturally important crop\nplants. The utilization of these databases in applied crop improvement program is still being achieved widely; otherwise, the end\nfor sequencing is not far away....
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